HansaBioMed Life Sciences 於 2007 年成立於愛沙尼亞，是最早專注於外泌體研究的公司之一，產品主要提供給 癌症研究、液體活檢、細胞間訊號研究等實驗室。
HansaBioMed Shop 產品整理
| 產品類別 | 代表產品 | 用途 | 技術核心 |
|---|---|---|---|
| Exosome / EV standards | Lyophilized Exosome Standards、Fluorescent EVs | 實驗標準品、方法驗證 | 標準化外泌體顆粒 |
| EV Isolation tools | EXO-Prep、miniPURE-EVs Spin | 分離外泌體 | precipitation / SEC |
| TFF filtration | TFF-Easy、TFF-EVs Small | 大體積濃縮 EV | Tangential Flow Filtration |
| SEC columns | PURE-EVs columns | 高純度分離 EV | Size Exclusion Chromatography |
| EV characterization | ExoTEST、Immunoplates | 定量、標誌蛋白分析 | ELISA-based capture |
| EV RNA isolation | EV RNA extraction kits | 外泌體 RNA 研究 | miRNA / mRNA |
| Antibodies | CD9 / CD63 / CD81 antibodies | EV marker detection | exosome markers |
| Fluorescent EVs | EGFP EVs | tracking / uptake study | fluorescent labeling |
| Plant EV / ELN | Seaberry ELNs | 植物來源 vesicles | nutraceutical / plant EV research |
1️⃣ Fluorescent EVs
- HEK293 cell engineered
- 約 1×10⁹ particles/vial
- 用於
- NTA calibration
- Flow cytometry
- cell uptake study
- imaging
2️⃣ PURE-EVs SEC column
從以下樣品純化 EV：
- plasma
- urine
- conditioned media
優點
- 比 precipitation 更乾淨
- 不需要 ultracentrifuge
3️⃣ TFF-Easy
- EV sample concentration + buffer exchange
技術：Tangential Flow Filtration
優點 1.scalable 2.保持 EV integrity
4️⃣ ExoTEST platform
| Product Category | Product Line | Example Product | Technology | Sample Type | Application | Key Features |
|---|---|---|---|---|---|---|
| EV Standards | Lyophilized Exosome Standards | Human plasma exosomes | TFF + SEC purified EVs | Plasma / serum / urine | Calibration / method validation | 高純度、凍乾保存穩定 |
| EV Standards | Plant EV standards | Seaberry ELNs | Natural plant EV isolation | Plant extract | EV functional research | >10¹⁰ particles/100µg |
| Fluorescent EVs | FLuoEVs | EGFP EVs / mCherry EVs | Genetic labeling | Cell derived EVs | Uptake / imaging / tracking | 螢光 EV 模型 |
| EV Isolation | EXO-Prep | Plasma / urine / media kits | Chemical precipitation | Biofluids / cell media | EV isolation | 單步驟分離 EV |
| EV Isolation | PURE-EVs | PURE-EVs columns | Size Exclusion Chromatography | Plasma / serum / urine / CM | EV purification | 約 15 min 完成分離 (hansabiomed.eu) |
| EV Isolation | miniPURE-EVs | Spin SEC column | SEC centrifugation | Small sample volume | Rapid EV purification | 小體積樣本 |
| EV Isolation | maxiPURE-EVs | Large SEC column | SEC | Large volume biofluids | High throughput purification | 高通量 |
| EV Isolation | EXO-FILTER | Ion exchange membrane | Ion exchange chromatography | Plasma / serum | EV enrichment | 正電膜捕捉 EV (hansabiomed.eu) |
| Immunocapture | Immunobeads | EV-IBeads | Antibody capture | Plasma / cell media | EV subtype capture | 磁珠免疫捕捉 |
| Immunocapture | Immunoplates | EV-IPlates | ELISA capture | Biofluids | EV detection | plate-based capture |
| EV Concentration | TFF-Easy | TFF-Easy concentrator | Tangential Flow Filtration | Diluted EV samples | EV concentration | buffer exchange |
| EV Separation | TFF-MV | TFF cartridge | TFF | EV / microvesicles | MV separation | >150 nm separation |
| EV Separation | TFF-EVs | Hollow fiber cartridge | TFF | EV isolation | purification | 中空纖維膜過濾 |
| Complete Kits | PURE-EVs PLUS | SEC + TFF system | TFF + SEC | Urine / cell media | High purity EV isolation | EV purity <1 hr |
| EV Characterization | ExoTEST | ExoTEST ELISA kits | ELISA detection | Biofluids / EV prep | EV marker detection | CD9 / CD63 / CD81 |
| EV Analysis | Exo-FACS | Flow cytometry kit | bead-based FACS | EV samples | EV phenotyping | multiparameter |
| EV Quantification | EV Quantification kit | particle / protein assay | biochemical quantification | EV prep | EV concentration measurement | standardization |
| RNA Extraction | EV RNA Basic Kit | RNA extraction | TRIzol-based | EV samples | miRNA / mRNA analysis | downstream sequencing |
| Antibodies | EV antibodies | CD9 / CD63 / CD81 | monoclonal antibodies | EV markers | Western / ELISA / FACS | exosome markers |
典型實驗 workflow
HansaBioMed 技術平台總覽
EV 研究其實可以濃縮為 4 大技術平台：
| Platform | Technology |
|---|---|
| Isolation | Precipitation / SEC |
| Purification | TFF / Ion exchange |
| Characterization | ELISA / FACS |
| Functional research | Fluorescent EV |
HansaBioMed 幾乎 涵蓋整條 EV workflow ，從分離 → 分析 → 標準品。
HansaBioMed Life Sciences EVs standards 凍乾標準品
細胞外囊泡（EVs）標準品皆透過切向流過濾（TFF）或粒徑篩析層析法（SEC）純化，並經過冷凍乾燥處理以利長期保存。
🔬 HansaBioMed EVs 凍乾標準品總表
| 產品分類 | 來源 / 細胞株 | 產品貨號 (Catalog No.) | 包裝規格與顆粒數 |
| 人體生物體液 (s-EVs / 外泌體) | 健康捐贈者尿液 (Human urine) | HBM-PEU-@/# | @ 劑量: 30 µg (>1×10⁸ 顆) 或 100 µg (>1×10¹⁰ 顆) # 瓶數: 2 瓶 或 5 瓶 |
| 健康捐贈者血漿 (Human plasma) | HBM-PEP-@/# | 同上 | |
| 健康捐贈者血清 (Human serum) | HBM-PES-@/# | 同上 | |
| 健康捐贈者血小板 (Human Platelets) | HBM-PET-100/# | 100 µg (>1×10¹⁰ 顆) / 2 或 5 瓶 | |
| 間質幹細胞 (MSCs) (s-EVs / 外泌體) | 人類脂肪衍生 MSC | HBM-MSC-100/# | 100 µg (>1×10¹⁰ 顆) / 2 或 5 瓶 |
| 細胞培養液 (s-EVs / 外泌體) | 常見細胞: HEK293, CHO 大腸癌: COLO1, HCT116, HT29 前列腺癌: PC3, LnCAP 肺癌: A549, NCI-H1975 神經膠質瘤/母細胞瘤: U87 MG, SK-N-SH 白血病: K562 乳癌: SK-BR-3, MCF7 胰臟癌: BxPC-3 小鼠黑色素瘤: B16F10 | HBM-[細胞簡稱]-@/# (例如: HBM-HEK293-@/#) | @ 劑量: 30 µg (>1×10⁸ 顆) 或 100 µg (>1×10¹⁰ 顆) # 瓶數: 2 瓶 或 5 瓶 |
| 細胞培養液 (MVs / 微囊泡) | HEK293, COLO1, NCI-H1975, U87 MG, SK-N-SH, HCT116, PC3, LnCAP, A549, K562, B16 | HBM-mv[細胞簡稱]-50 (例如: HBM-mvHEK-50) | 50 µL (>1×10⁹ 顆) |
| 植物類外泌體 (ELNs) | 生薑 (Ginger root) 馬鈴薯 (Potato) 洋蔥 (Onion) 大蒜 (Garlic) 沙棘果 (Seaberries) | HBM-ELN-GIN-100 HBM-ELN-POT-100 HBM-ELN-ONI-100 HBM-ELN-GAR-100 HBM-ELN-SEA-100 | 100 µg (>1×10¹⁰ 顆) |
| 螢光表現外泌體 (FLuoEVs) | HEK293 融合螢光蛋白表現： 綠色 EGFP (CD63, CD81, CD9) 紅色 mCherry (CD63, CD81, CD9) 藍色 BFP (CD81) 雙色 EGFP + mCherry | HBM-HEK-EGFP63 HBM-HEK-mCH81 (以此類推) | 1×10⁹ 個螢光顆粒 / 瓶 |
| 螢光染色外泌體 | 多種來源外泌體 (Bodipy 染色) | HBM-F-#### | 100 µL (>1×10⁹ 顆) |
| 螢光染色微囊泡 | 多種來源微囊泡 | HBM-MVF-#### | 50 µL (>1×10⁹ 顆) |
💡 產品代碼說明：
- 貨號中的
@代表容量規格：可選擇 30 (30 µg) 或 100 (100 µg)。 - 貨號中的
#代表包裝瓶數：可選擇 2 (兩瓶裝) 或 5 (五瓶裝)。 - 舉例：
HBM-PEU-30/2代表「人類尿液外泌體標準品，每瓶 30 µg，共 2 瓶裝」。